How to use

practical advice from aesthetic professionals and beauty gurus

DefenAge skincare routine

by Victoria Lewis, Medical Aesthetician

Tribeca MedSpa

A treat for skin before going to bed

by Amy Forman Taub, MD, Dermatologist

Advanced Dermatology

How to open DefenAge’s bottle

by DefenAge




Product Use

How fast can I see results?

Initial results can be seen in as little as 1 week, full range of results – 6 weeks.

Why are a few days enough to see the first results with DefenAge?
It is enough because our unique cosmetic ingredient, the defensin-molecules, works together with the same “fresh” skin master cells that our body uses to heal wounds. Our skin heals scratches within a few days, meaning that it is enough for the body to produce a visible effect that fast.

TIP: Exfoliate skin to see results faster.
Remove old cells from the skin's surface to make new skin visible using an exfoliating mask. It also helps keep pores clean. DefenAge's exfoliation solution is the 2-Minute Reveal Masque, part of all our kits. It will take only 2 minutes, once or twice a week.
Can I combine DefenAge  with Retin-A or other skincare?

Yes, you can! This is a unique property of defensin-molecules, our core anti-aging technology. Due to an entirely different mechanism of action, DefenAge can be combined with almost any other skincare products and ingredients, including Retin-A and retinols, vitamin C, hyaluronic acid, and growth factors. The rule is simple: layer all other products in your skincare routine before layering DefenAge's Step 2 and 3.

How do I include DefenAge in my skincare routine with Retin-A, vit C etc?
The rule is simple: Apply Step 2 and Step 3 at the very end! DefenAge's Step 2 and 3 should be the last steps in your skincare routine; only sunscreen and makeup should be layered on top of our bioserum in the morning and nothing at night.
This is the order for using the products that we recommend:
STEP 1 - Cleansing:
Facial cleanser, morning and evening. Please use any cleanser you like or DefenAge's 1-Step Multi-Cleanse. Expholiate the skin after cleansinig with 2-Minute Reveal Masque once or twice a week.
Any other skincare products that you would like to use, including vitamin C, Retin A/retinols etc.
STEP 2 - Hydration:
FACE: 24/7 Barrier Balance Cream on the face, except the skin around eyes; morning and evening.
EYES: 3D Eye Radiance Cream for the skin around eyes; morning and evening.
NECK: 6-Week Perfection Neck Tightening Cream for the skin on the neck; morning and evening.
STEP 3 - New Skin:
8-in-1 BioSerum Anti-Aging Cream on the entire face including the skin around eyes and on the neck; morning and evening.
SUNSCREN and MAKEUP in the morning.
Can I apply more product for more results?
Please use the exact quantity of the defensin products as we recommend: one pump per area (one pump rule). Do not overdose, more is not better. Our formulas are very well balanced and refined on concentrations of defensins and other ingredients to deliver results.
24/7 Barrier Balance Cream: ONE (up to two) pumps on the face.
3D Eye Radiance Cream: ONE pump for both eyes.
6-Week Perfection Neck Tightening Cream: ONE pump on the neck and décolleté.
8-in-1 BioSerum: ONE pump on the face and ONE pump on the neck and décolleté.
Why should STEP 2 creams be layered before 8-in-1 BioSerum?

Together with the activity to stimulate new skin, the defensin-molecules have an extra beneficial activity to "close gaps" between cells on the surface of our skin. It means that defensins prevent water loss from the skin. The 8-in-1 BioSerum contains the highest concentration of defensins, meaning that once our bioserum is applied, penetration into the skin surface other products will be compromised (if "nothing/water goes out" - then "nothing is goes in").

This is a unique property of defensin molecules, therefore we always advise layering the 8-in-1 BioSerum as the last step of the skincare regimen, only sunscreen and makeup (if any) is recommended to be applied on top of the 8-in-1 BioSerum in the morning or nothing at night.


Is DefenAge safe during pregnancy and breastfeeding?

We built our formulas with clean beauty in mind, our formulas are clean and do not contain most common harmful or questionable ingredients. In addition, please know that our formulas do not contain retinols. Unfortunately, we do not have formal clinical studies relating to safety during pregnancy and breastfeeding, therefore, before you start using DefenAge, we advise to consult the ingredient list with your OBGYN to be double sure. The detailed ingredient lists are shown on the product pages of our website.

Is 8-in-1 BioSerum good for acne prone skin?

Yes it is. The 8-in-1 BioSerum is safe for acne prone skin, our bio serum underwent multiple safety studies that returned an outstanding safety profile. Please be sure that you use the exact quantity of the product that we recommended, and do not exceed it: 1 pump of the bio serum for entire face and 1 pump on the neck.

Science and Technology

What are defensins and what do they do?
  • Defensins are natural messaging molecules found in our body.
  • Defensins do not cause inflammation or sun-sensitivity.
  • Defensins are produced in the skin by our immune cells, neutrophils.
  • DefenAge contains an identical copy of human defensins made via bio-synthesis.


The age-repairing nature of these natural molecules was discovered by scientists at DefenAge® while researching cell therapies for wound healing and Type I Diabetes. During the course of their research, our scientific team made an extraordinary discovery: two members of the defensin family have surprising benefits for aging skin in cosmeceutical skincare. Alpha-defensin 5 communicates specifically with Skin Master Cells to unlock the skin’s age-corrective potential. Beta-defensin 3 significantly improves skin’s barrier functions. This discovery was so novel and unexpected we are patenting the use of these molecules for anti-aging purposes.

Read more about defenins in the Defensins eBook, click here.

Why do dermatologists recommend defensins?

Let us share a few links:

Defensins-technology and DefenAge: Video review by Penny Smith, Master Aesthetician, Portland, OR. To watch the video on Penny Smith' channel click here.

Science and clinical studies behind defensins: Video webinar by Amy Taub, MD, Dermatologist, Lincolnshire, IL. To watch the video on Dr. Taub's channel click here.

What is Clean Beauty in DefenAge?

Please visit our new Clean Beauty Page, we have all the answers there!


Why should animal- and human- derived ingredients be a concern?

Because such ingredients bring safety risk, we screen our ingredients to remove any potential risk that may come with using human or animal-sourced products. By excluding such sources, we remove the possibility of infections and diseases that can be traced to living cells and tissues, so we do not use growth factors or cell media produced by living cells nor do our products contain any animal or human cells, stem cells, blood cells, placental cells, other tissues or any substances that they may produce. Instead, we rely on advanced and safe alternative bio-sources and standards used in pharmaceutical manufacturing. DefenAge, through these strict rules, has raised the expectations of safety in skincare and cosmetics by being "free from animal- and human-derived ingredients."

Is DefenAge safe?

All products made by DefenAge® are tested on humans, not other animals. We do not believe animal studies are accurate enough to determine human safety. Nor are they ethical.

To prove safety we put our products through a rigorous series of clinical tests. Among these are:

  • 30-Day HRIPT (Human Repeat Insult Patch Test) to determine irritancy and allergy potential of a leave-on product. Results are reviewed and signed by a board certified dermatologist.
  • 14-Day Clinical Evaluation of Ophthalmologic Safety for all products used around the eye. Products are tested for safe use around the eye and for safe use by contact lens wearers. Results are reviewed and signed by a board certified ophthalmologist.


How to Save • Free Account • Shipping

How do I save with DefenAge?

We recommend making purchases after logging in to your account. After login, prices will be adjusted automatically. An account at DefenAge is FREE and does not require a subscription or purchase commitment, but it gives 25% savings on almost everything! If you do not have an account yet, create one here.

How to get 25% off promo code?

There is no need for a promo code; you should simply need to log in to your account at defenage.com, prices will be adjusted automatically. If you don't have an account, you can create one here: https://defenage.com/customer/account/create/
The account at DefenAge is free and does not require purchase commitment or subscription.

Is DefenAge sold in drugstores?

DefenAge is only available on defenage.com or DefenAge-authorized aesthetic practices and their websites. DefenAge is not available in drugstores.

Can I buy DefenAge on Amazon or eBay?

Authentic DefenAge® products are not available on Amazon or eBay.

Do you ship internationally?

Unfortunately, we do not ship internationally at this point. But please stay tuned - we may start shipping to your region soon! Please consider subscribing to our e-mails to get instant updates. You can subscribe on this page: https://defenage.com/contact-us


CLINICAL POWER TRIO clinical study tested, DefenAge’s core skin care regimen